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Closed Access item Studies on the Translation of the Genetic Message. II. Translation of Oligonucleotide Messengers of Specified Base Sequence

Authors:Wahba, A. J.
Salas, Margarita
Stanley, W. M.
Keywords:Lactobacillus arabinosus, E. coli, Protein synthesis, Ribosomes
Issue Date:1966
Publisher:Cold Spring Harbor Laboratory. Press
Citation:Cold Spring Harbor Symposia on Quantitative Biology 31: 103-111 (1966)
Abstract:Studies on the translation of the genetic message, using synthetic polyribonucleotides as messengers in the conventional cell-free system of Escherichia coli for protein synthesis, have been seriously hindered by (a) the presence of nucleases and peptidases that degraded messengers and polypeptides, respectively, and (b) the difficulty of performing end-group assays on the insoluble products formed. These obstacles have been removed through (a) the use of a system low in nuclease activity consisting of chromatographically purified E. coli ribosomes and a supernatant fraction derived from Lactobacillus arabinosus, and (b) the preparation and unequivocal characterization of adenylic acid-rich oligonucleotides of specified base sequence. These synthetic messengers direct the synthesis of lysine-containing peptides which are soluble in water and can be readily characterized.
Publisher version (URL):http://dx.doi.org/10.1101/SQB.1966.031.01.017
URI:http://hdl.handle.net/10261/47132
ISSN:0091-7451
E-ISSNmetadata.dc.identifier.doi = DOI:1943-4456
Appears in Collections:(CBM) Artículos

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