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Closed Access item Effect of polyadenylic acid chain length on the size distribution of lysine peptides.

Authors:Smith, M. A.
Salas, Margarita
Stanley, W. R.
Wahba, A. J.
Ochoa, Severo
Keywords:E.coli, Amino acid, L. arabinosus, length
Issue Date:1966
Publisher:Polskie Towarzystwo Biochemiczne
Citation:Acta Biochimica Polonica 13(4): 361-365
Abstract:1, Purified E. coli ribosomes d L. arabinosus supenatant were used in an amino acid incorporating system to study the effect of polyadeny1ic acid chain length on the size distributions of the lysine peptides formed. 2, Oligoadenylic acids of increasing chain length were preparad by partial alkaline hydrolysis of polyadenylic acid, followed by treatment with acid and phosphomonoesterase, and chromatography in 8 M-urea an DEAE-cellulose. 3, The size distribution of lysine peptides was determined by chromatography on carboxymethyl cellulose. 4. The most abundant peptide formed with each oligoadenylic acid of chain length between 12 and 20 indicated a triplet code.
Publisher version (URL):http://www.ncbi.nlm.nih.gov/pubmed/5336165
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