Biología y Biomedicina >
Centro de Biología Molecular Severo Ochoa (CBM) >
(CBM) Artículos >
Closed Access item Effect of polyadenylic acid chain length on the size distribution of lysine peptides.
|Authors:||Smith, M. A.|
Stanley, W. R.
Wahba, A. J.
|Keywords:||E.coli, Amino acid, L. arabinosus, length|
|Publisher:||Polish Biochemical Society|
|Citation:||Acta Biochimica Polonica 13(4): 361-365|
|Abstract:||1, Purified E. coli ribosomes d L. arabinosus supenatant were used in an amino acid
incorporating system to study the effect of polyadeny1ic acid chain length on the size
distributions of the lysine peptides formed.
2, Oligoadenylic acids of increasing chain length were preparad by partial alkaline hydrolysis of polyadenylic acid, followed by treatment with acid and phosphomonoesterase, and chromatography in 8 M-urea an DEAE-cellulose.
3, The size distribution of lysine peptides was determined by chromatography on carboxymethyl cellulose.
4. The most abundant peptide formed with each oligoadenylic acid of chain length between 12 and 20 indicated a triplet code.|
|Publisher version (URL):||http://www.ncbi.nlm.nih.gov/pubmed/5336165|
|Appears in Collections:||(CBM) Artículos|
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.