Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/46558
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | A Mutant D-Fructose-6-Phosphate Aldolase (Ala129Ser) with Improved Affinity towards Dihydroxyacetone for the Synthesis of Polyhydroxylated Compounds |
Autor: | Castillo Expósito, José Antonio CSIC; Guérard-Hélaine, Christine; Gutiérrez, Mariana CSIC; Garrabou, Xavier CSIC ORCID; Sancelme, Martine; Schürmann, Melanie; Inoue, Tomoyuki; Hélaine, Virgil; Charmantray, Franck; Gefflaut, Thierry; Hecquet, Laurence; Joglar Tamargo, Jesús CSIC ORCID; Clapés Saborit, Pere CSIC ORCID ; Sprenger, Georg A.; Lemaire, Marielle | Palabras clave: | Ala129Ser mutant Carbohydrates D-fructose-6-phosphate aldolase Dihydroxyacetone Nitrocyclitols |
Fecha de publicación: | 2010 | Editor: | Wiley-VCH | Citación: | Advanced Synthesis and Catalysis | Resumen: | A mutant of D-fructose-6-phosphate aldolase (FSA) of Escherichia coli, FSA A129S, with improved catalytic efficiency towards dihydroxyacetone (DHA), the donor substrate in aldol addition reactions, was explored for synthetic applications. The kcat/KM value for DHA was 17-fold higher with FSA A129S than that with FSA wild type (FSA wt). On the other hand, for hydroxyacetone as donor substrate FSA A129S was found to be 3.5-fold less efficient than FSA wt. Furthermore, FSA A129S also accepted glycolaldehyde (GA) as donor substrate with 3.3-fold lower affinity than FSA wt. This differential selectivity of both FSA wt and FSA A129S for GA makes them complementary biocatalysts allowing a control over donor and acceptor roles, which is particularly useful in carboligation multi-step cascade synthesis of polyhydroxylated complex compounds. Production of the mutant protein was also improved for its convenient use in synthesis. Several carbohydrates and nitrocyclitols were efficiently prepared, demonstrating the versatile potential of FSA A129S as biocatalyst in organic synthesis. | Versión del editor: | http://dx.doi.org/10.1002/adsc.200900772 | URI: | http://hdl.handle.net/10261/46558 | DOI: | 10.1002/adsc.200900772 | ISSN: | 1615-4150 | E-ISSN: | 1615-4169 |
Aparece en las colecciones: | (IQAC) Artículos |
Mostrar el registro completo
CORE Recommender
SCOPUSTM
Citations
86
checked on 20-mar-2024
WEB OF SCIENCETM
Citations
82
checked on 24-feb-2024
Page view(s)
427
checked on 28-mar-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.