Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/45813
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Título : Effect of Early Introduction of Microencapsulated Diet to Larval Atlantic Halibut, Hippoglossus hippoglossus L. Assessed by Microarray Analysis
Autor : Murray, Harry M., Lall, S. P., Rajaselvam, R., Boutilier, L. A., Flight, R. M., Blanchard, B., Colombo, S., Mohindra, V., Yúfera, Manuel, Douglas, Susan E.
Palabras clave : Microencapsulated diet
Larvae
Atlantic halibut
Microarray
Fecha de publicación : 18-Jul-2009
Editor: National Research Council Canada
Resumen: An experimental microdiet prepared using an internal gelation method was used to partially replace the traditional live feed (Artemia) for larval Atlantic halibut, Hippoglossus hippoglossus L. Three trials were conducted with microdiet introduced at 20, 32, and 43 days post first feeding and larvae were sampled at approximately 2, 13, 23, and 33 days after microdiet introduction in each trial. The success of feeding was assessed by morphometrics and histological analysis of gut contents. Microdiet particles were readily consumed after a period of adaptation and provided an adequate source of nutrients with no significant increase in mortality in the microdiet-fed group compared to the control group. However, growth was limited and there was an increased incidence of malpigmentation of the eye and skin. Subtle changes in underlying digestive and developmental physiology were revealed by microarray analysis of RNA from control and experimental fish given microdiet from day 20 post first feeding. Fifty-eight genes were differentially expressed over the four sampling times in the course of the trial and the 28 genes with annotated functions fell into five major categories: metabolism and biosynthesis, cell division and proliferation, protein trafficking, cell structure, and stress. Interestingly, several of these genes were involved in pigmentation and eye development, in agreement with the phenotypic abnormalities seen in the larvae.
Descripción : 16 páginas, 8 tablas, 5 figuras.
Versión del editor: http://dx.doi.org/10.1007/s10126-009-9211-4
URI : http://hdl.handle.net/10261/45813
ISSN: 1436-2228
DOI: 10.1007/s10126-009-9211-4
Citación : Marine Biotechnology 12(2): 214-229 (2009)
Referencias: PMID: 19618242
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