English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/45288
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Citado 13 veces en Web of Knowledge®  |  Pub MebCentral Ver citas en PubMed Central  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar bibText (RIS)Exportar csv (RIS)
Título

Using a dual-stable isotope tracer method to study the uptake, xylem transport and distribution of Fe and its chelating agent from stereoisomers of an Fe(III)-chelate used as fertilizer in Fe-deficient Strategy I plants

Autor Orera Utrilla, Irene ; Rodríguez-Castrillón, José Ángel; Moldovan, Mariella; García-Alonso, José I.; Abadía Bayona, Anunciación ; Abadía Bayona, Javier ; Álvarez-Fernández, Ana
Fecha de publicación ago-2010
EditorRoyal Society of Chemistry (Great Britain)
Citación Orera I, Rodríguez-Castrillón JA, Moldovan M, García-Alonso JI, Abadía A, Abadía J, Álvarez-Fernández A. Using a dual-stable isotope tracer method to study the uptake, xylem transport and distribution of Fe and its chelating agent from stereoisomers of an Fe(III)-chelate used as fertilizer in Fe-deficient Strategy I plants. Metallomics 2: 646-657 (2010)
ResumenA dual-stable isotope tracer experiment was carried out with Fe-deficient sugar beet plants grown hydroponically and resupplied with differentially Fe labeled racemic and meso Fe(III)-chelates of the ethylendiamine di(o-hydroxyphenylacetic) acid (o,oEDDHA). No short-term Fe isotope exchange reactions occurred in the nutrient solution and plants did not discriminate between 54Fe and 57Fe. After 3–6 h, stable Fe isotopes, chelating agents and chelates were analyzed in roots, xylem sap and leaves by ICP-MS and HPLC-ESI/TOFMS. Ferric chelate reductase rates, xylem transport and total uptake were 2-fold higher with the meso isomer than with the racemic one. Both chelating agent isomers were incorporated and distributed by plants at similar rates, in amounts one order of magnitude lower than those of Fe. After 6 h of Fe resupply, most of the Fe acquired was localized in roots, whereas most of the chelating agent was in leaves. In a separate experiment, Fe-deficient sugar beet and tomato plants were treated with different concentrations of Fe(III)-o,oEDDHA (with a meso/racemic ratio of 1). The xylem sap Fe concentration at 24 h was unaffected by the chelate concentration, with xylem Fe(III)-o,oEDDHA accounting for 1–18% of total Fe and xylem meso/racemic ratio close to 1. Although most of the Fe coming from Fe(III)-o,oEDDHA was taken up through a reductive dissociative mechanism, a small part of the Fe may be taken up via non-dissociative mechanisms.
Descripción 12 Pags., 1 Tabl., 8 Figs.
Versión del editorhttp://dx.doi.org/10.1039/c0mt00018c
URI http://hdl.handle.net/10261/45288
DOI10.1039/C0MT00018C
ISSN1756-5901
E-ISSN1756-591X
Aparece en las colecciones: (EEAD) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
mod vers.aut pend.pdf20,83 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 



NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.