English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/44709
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar bibText (RIS)Exportar csv (RIS)
Título

Parthenogenic Haploids in Melon: Generation and Molecular Characterization of a Doubled Haploid Line Population

Autor Gonzalo Pascual, María José ; Claveria Capaul, Elisabeth ; Monforte, Antonio J.; Dolcet-Sanjuán, Ramón
Palabras clave Cucurbitaceae
parthenogenesis
pure line
hybrid
DHL
markers
segregation distortion
Fecha de publicación 2011
EditorAmerican Society for Horticultural Science
Citación Gonzalo MJ, Clavería E, Monforte AJ, Dolcet-Sanjuán. Parthenogenic Haploids in Melon: Generation and Molecular Characterization of a Doubled Haploid Line Population. Journal of the American Society for Horticultural Science 136 (2): 145–154 (2011)
ResumenMelon (Cucumis melo) is one of the principal vegetable crops for fresh market, for which a large number of breeding programs, oriented to generate inbred pure lines and hybrids, is established worldwide. The process to obtain and select these lines has been highly accelerated by the use of biotechnological techniques such as the generation of doubled haploid line (DHL) populations and molecular markers. Moreover, the use of DHLs in genetic studies is a useful tool because of their complete homozygosity and the permanent availability of plant material perpetuated by seed. In this work, the parthenogenetic response of 17 melon genotypes and the F1 hybrid PI 161375 × Spanish cultivar Piel de Sapo (PS) was studied considering three stages along the in vitro DHL generation process. The response of the analyzed melon cultivars was heterogeneous through the DHL generation with different limiting steps for each genotype. The response of the PI 161375 × PS hybrid was more similar to the male (PS) than the female parent (PI 161375), although the response of the maternal genotype was higher for some stages. This points to the important role of alleles from both parents in the different steps of the DHL generation process, and it could explain the identification of six genomic regions with distorted allelic segregation skewed toward PS or PI 161375. This hybrid was used to generate a population of 109 DHLs, the gametophytic origin of which was confirmed by flow cytometry and molecular markers.
Descripción 10 Pags., 3 Tabls., 4 Figs.
Versión del editorhttp://journal.ashspublications.org/content/136/2/145.full
URI http://hdl.handle.net/10261/44709
ISSN0003-1062
Aparece en las colecciones: (EEAD) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
revista no permitida - white.pdf37,14 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.