English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/44377
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:


Hydrolysis optimization of mannan, curdlan and cell walls from Endomyces fibuliger grown in mussel processing wastewaters

AuthorsPrieto, Miguel Ángel ; Vázquez, José Antonio ; Murado García, Miguel Anxo
KeywordsYeast cell walls
Mussel processing wastes
Endomyces fibuliger
Trifluoroacetic acid hydrolysis
Mathematical modeling
Curdlan and mannan
Issue Date10-May-2011
CitationProcess Biochemistry 46(8): 1579–1588 (2011)
AbstractThe aim of this report was to optimize the hydrolysis of the cell walls (CWs) from the yeast Endomyces fibuliger grown in mussel processing wastewaters (MPW) to establish a more accurate protocol for analyzing the composition of the monosaccharides in these CWs. Therefore, a kinetic study of CW hydrolysis and polysaccharide standards (mannan and curdlan) was performed to determine the effect of different temperatures and trifluoroacetic acid (TFA) concentrations on this process. In all cases, the experimental data were fit satisfactorily to Saeman's equation with an Arrhenius relation between rate constants and the temperature effect. Optimal conditions for curdlan and mannan hydrolysis were achieved with 70% TFA at 100 °C for 2.3 h and 50% TFA at 100 °C for 2.6 h, respectively. The best operating options for CW hydrolysis were 100 °C/70% TFA for 4.58 h, 100 °C/50% TFA for 4.08 h and 100 °C/70% TFA for 3.27 h for the maximum production of glucose, mannose and reducing sugars, respectively.
Publisher version (URL)http://dx.doi.org/10.1016/j.procbio.2011.04.014
Appears in Collections:(IIM) Artículos
Files in This Item:
File Description SizeFormat 
Prieto1.pdf326,41 kBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.