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Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/44223
Title: Molecular characterization and expression analysis of the putative interleukin 6 receptor (IL-6Rα and glycoprotein-130) in rainbow trout (Oncorhynchus mykiss): salmonid IL-6Rα possesses a polymorphic N-terminal Ig-domain with variable numbers of two repeats
Authors: Costa, M. M. ; Wang, T.; Monte, Milena M.; Secombes, Christopher J.
Keywords: Interleukin-6
Phylogenetic analysis
Repeat number polymorphism
Issue Date: 2012
Publisher: Springer
Citation: Immunogenetics 64(3) : 229-244 (2012)
Abstract: Interleukin (IL)-6, the founding member of IL-6 family cytokines, plays non-redundant roles in hematopoiesis and acute phase responses. IL-6 signals via a specific private IL-6Rα and a common beta chain gp130. In this study, we sequence analysed both IL-6Rα and gp130 in rainbow trout. The trout gp130 cDNA encodes 906 aa and is similar in size, extracellular domain structure (D1-6) and presence of intracellular motifs important for signal transduction to tetrapod gp130. The trout IL-6Rα cDNA encodes for 834 aa and is larger compared to tetrapod IL-6Rαs, as are other fish IL-6Rα molecules due to a large D1 domain. However, the cytokine binding domain is well conserved across vertebrates, with four conserved cysteine residues in the N-terminal FNIII domain and a WSXWS motif in the C-terminal FNIII domain. Furthermore, phylogenetic tree analysis confirmed that the reported fish IL-6Rα and gp130 molecules are orthologues to their tetrapod counterparts. The extra-large D1 domain of the salmonid IL-6Rα molecules results from the insertions of two repetitive sequences of [TS]-[TF]-VSTTT-[ND]-TTSNG and TTVS-[AT]-IKD-[DG]-S-[KD]-N-[GR], respectively. Furthermore the numbers of repetitions of the two motifs were variable in different individuals and cell lines, and even in the same fish allelic polymorphism exists. Trout IL-6Rα was expressed at higher levels than gp130 in a number of tissues examined and the expression of both IL-6Rα and gp130 could be modulated by LPS and Poly I:C in four trout cell lines studied. The expression patterns of the receptors suggest that high level expression of IL-6Rα is critical for IL-6 responsiveness.
Description: 16 páginas, 6 figuras, 3 tablas.-- The final publication is available at www.springerlink.com
Publisher version (URL): http://dx.doi.org/10.1007/s00251-011-0581-1
URI: http://hdl.handle.net/10261/44223
DOI: 10.1007/s00251-011-0581-1
E-ISSN: 0093-7711
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