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Título

Characterization of flavins in roots of Fe-deficient strategy I plants, with a focus on Medicago truncatula

AutorRodríguez-Celma, Jorge ; Vázquez-Reina, Saúl ; Orduna, Jesús; Abadía Bayona, Anunciación ; Abadía Bayona, Javier ; Álvarez-Fernández, Ana ; López-Millán, Ana Flor
Palabras claveIron deficiency
Legume
Mass spectrometry
Medicago truncatula
Riboflavin
Riboflavin derivative identification
Root
Fecha de publicacióndic-2011
EditorOxford University Press
CitaciónPlant and Cell Physiology 52(12): 2173-2189 (2011)
ResumenThe root accumulation and excretion of riboflavin (Rbfl) and Rbfl derivatives have been studied in the model legume species Medicago truncatula, grown in hydroponics in two different Fe deficiency conditions, with and without CaCO3. Using high resolution mass spectrometry techniques coupled to liquid chromatography, three different flavin derivatives not previously reported in plants, putatively identified as 7-hydroxy-Rbfl, 7a-hydroxy-Rbfl and 7-carboxy-Rbfl, were found along with Rbfl in Fe-deficient M. truncatula roots. In the presence of CaCO3 most of the flavins were accumulated in the roots, whereas in the absence of CaCO3 there was partial export to the nutrient solution. The major flavins in roots and nutrient solution were Rbfl and 7-hydroxy-Rbfl, respectively. Flavins were located in the root cortex and epidermal cells, preferentially in a root region near the apex that also exhibited increased ferric chelate reductase (FCR) activity. Six out of 15 different species of horticultural interest showed root increases in both Rbfl (four of them also having Rbfl derivatives) and FCR. No significant correlation was found between Rbfl and either phosphoenolpyruvate carboxylase or FCR activities, whereas the latter two showed a good correlation between them. The possible roles of Rbfl and Rbfl derivatives in roots and nutrient solutions are discussed. Medicago truncatula is proposed as a model system for flavin studies.
Descripción17 páginas, 7 figuras, 3 tablas.
Versión del editorhttp://dx.doi.org/10.1093/pcp/pcr149
URIhttp://hdl.handle.net/10261/43963
DOI10.1093/pcp/pcr149
ISSN0032-0781
E-ISSN1471-9053
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