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dc.contributor.authorCerdá-Reverter, José Miguel-
dc.contributor.authorMartínez-Rodríguez, Gonzalo-
dc.contributor.authorZanuy, Silvia-
dc.contributor.authorCarrillo, Manuel-
dc.contributor.authorLarhammar, Dan-
dc.date.accessioned2011-12-15T07:49:18Z-
dc.date.available2011-12-15T07:49:18Z-
dc.date.issued1999-06-09-
dc.identifier.citationComparative Biochemistry and Physiology - B - Biochemistry and Molecular Biology 123(2): 181-186 (1999)es_ES
dc.identifier.issn1096-4959-
dc.identifier.otherPMID: 10425721-
dc.identifier.urihttp://hdl.handle.net/10261/43348-
dc.description6 páginas, 4 figuras.es_ES
dc.description.abstractA partial genomic library of sea bass DNA was constructed and screened with a goldfish NPY cDNA probe. Two identical clones were isolated and sequenced. The clones contain a segment with high identity to exon 2 of the NPY gene in tetrapods. This segment encodes a 62-amino acid peptide consisting of a signal peptide of 28 amino acids and the main portion of the mature NPY (34 amino acids). In the latter extension, sea bass NPY shows high identity with the human and deduced ancestral gnathostome sequences (88 and 91%, respectively). The open reading frame is followed by a consensus splice donor site. Northern blot hybridization to examine tissue distribution detected a 1-kb RNA transcript restricted to brain tissue. These data show that the NPY gene of this teleost fish has the same intron positions as tetrapods for at least two of the gene’s three introns. In addition, the high evolutionary conservation of NPY is corroborated since sea bass NPY exhibits the same identity to both goldfish and human NPY.es_ES
dc.description.sponsorshipJMCR received a grant from Balaguer-Gonel Foundation. GMR was supported by the ‘Programa de Contratación Temporal de Investigadores de MEC’. This work has been supported by a CICYT Project no.: MAR95-1888-CO3-01 to S.Z.es_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.rightsclosedAccesses_ES
dc.subjectNPYes_ES
dc.subjectPPes_ES
dc.subjectPancreatic polypeptidees_ES
dc.subjectDNA sequenceses_ES
dc.subjectGenomic DNAes_ES
dc.subjectGene expressiones_ES
dc.subjectBraines_ES
dc.subjectSea basses_ES
dc.subjectFishes_ES
dc.subjectMolecular evolutiones_ES
dc.titleCloning the neuropeptide Y Exon 2 from sea bass (Dicentrarchuslabrax)es_ES
dc.typeartículoes_ES
dc.identifier.doi10.1016/S0305-0491(99)00055-3-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1016/S0305-0491(99)00055-3es_ES
dc.identifier.e-issn1879-1107-
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