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Open Access item Conjugation of a Novel Apaf-1 Inhibitor to Peptide-based Cell-Membrane Transporters. Effective Methods to Improve Inhibition of Mitochondria-Mediated Apoptosis

Authors:Orzáez, Mar
Mondragón Martínez, Laura
Marzo, Isabel
Sanclimens Pérez de Rozas, Gloria
Messeguer Peypoch, Àngel
Pérez-Payá, Enrique
Vicent, María J.
Keywords:Apoptosis, Apoptosome inhibitors, Cell-penetrating peptides, Drug delivery systems, Conformational analysis, Membrane mimetics
Issue Date:May-2007
Citation:Peptides 28 (5): 958-968
Abstract:We have identified a family of peptoids that inhibits in vitro the activity of the apoptosome, a macromolecular complex that activates mitochondrial-dependent apoptosis pathways. The analysis of peptide-based cell compatible delivery systems of the most active peptoid is presented. The active peptoid was then fused to cell penetrating peptides (CPP) as penetratin (PEN-peptoid) and HIV-1 TAT (TAT-peptoid). PEN-peptoid showed greater cell viability and as a consequence better efficiency as an apoptosis inhibitor than the TAT-peptoid. The intracellular trafficking of both inhibitors was studied by flow cytometry and confocal fluorescence microscopy. Finally, the influence of the cargo (peptoid) molecules on the conformational behavior of the CPP in buffers and in membrane mimetic environments was analyzed using circular dichroism (CD) spectroscopy.
Description:The definitive verison is available at http://www.sciencedirect.com/science//journal/01969781
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