English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/43085
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Characterization of a partial α-amylase clone from red porgy (Pagrus pagrus): Expression during larval development

AuthorsDarias, M. J. ; Murray, Harry M.; Gallant, Jeffrey W.; Astola, A. ; Douglas, Susan E.; Yúfera, Manuel ; Martínez-Rodríguez, Gonzalo
KeywordsRed porgy
Pagrus pagrus
α-amylase expression
In situ hydridization
RT-PCR
Development
Digestive physiology
Fish larvae
Pancreas
Issue Date18-Jan-2006
PublisherElsevier
CitationComparative Biochemistry and Physiology - B - Biochemistry and Molecular Biology 143(2): 209-218 (2006)
AbstractA partial α-amylase cDNA was isolated from red porgy (Pagrus pagrus, Teleostei: Sparidae) and its tissue specific expression during larval development was examined. The cDNA was 949 bp long and showed 90% identity with other fish amylases. A 545 bp fragment was used to study amylase expression using in situ hybridization and RT-PCR techniques. Both methods showed a similar pattern: high and relatively constant expression for the first 30 days after hatching (dah), subsequently decreasing until the end of the experiment at 60 dah. The goal of this work was to extend the existing knowledge of the functionality of larval fish digestive systems and to provide new information about α-amylase gene expression.
Description10 páginas, 6 figuras, 2 tablas.
Publisher version (URL)http://dx.doi.org/10.1016/j.cbpb.2005.11.010
URIhttp://hdl.handle.net/10261/43085
DOI10.1016/j.cbpb.2005.11.010
ISSN1096-4959
E-ISSN1879-1107
Appears in Collections:(ICMAN) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.