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Closed Access item Two homologous low temperature inducible genes from Arabidopsis encode highly hydrophobic proteins

Authors:Capel, Juan
Jarillo, José Antonio
Salinas, Julio
Martínez-Zapater, José M.
Issue Date:1997
Publisher:American Society of Plant Biologists
Citation:Plant Physiology 115: 569-576 (1997)
Abstract:We have characterized two related cDNAs (RCIM and RC12B) corresponding to genes from Arabidopsis thaliana, the expression of which i s transiently induced by low, nonfreezing temperatures. R C l Z A and R C D B encode small(54 amino acids), highly hydropho- bic proteins that bear two potential transmembrane domains. They show similarity to proteins encoded by genes from barley (Hordeum vulgare L.) and wheatgrass (Lophophyrum elongatum) that are reg- ulated by different stress conditions. Their high leve1 of sequence homology (78%) and their genomic location in a single restriction fragment suggest that both genes originated as a result of a tandem duplication. However, their regulatory sequences have diverged enough to confer on them different expression patterns. Like most of the cold-inducible plant genes characterized, the expression of RC12A and R C / Z B i s also promoted by abscisic acid (ABA) and dehydration but i s nota general response to stress conditions, since it i s not induced by salt stress or by anaerobiosis. Furthermore, low temperatures are able to induce RC12A and RC12B expression in ABA-deficient and -insensitive genetic backgrounds, indicating that both ABA-dependent and 4ndependent pathways regulate the low- temperature responsiveness of these two genes.
Publisher version (URL):http://dx.doi.org/10.1104/pp.115.2.569
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