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Título : Comparative gene expression of gonadotropins (FSH and LH) and peptide levels of gonadotropin-releasing hormones (GnRHs) in the pituitary of wild and cultured Senegalese sole (Solea senegalensis) broodstocks
Autor : Guzmán, José M., Rubio, M., Ortiz-Delgado, Juan B., Klenke, U., Kight, Katherine, Cross, Ismael, Sánchez-Ramos, I., Riaza, A., Rebordinos, Laureana, Sarasquete, Carmen, Mañanós, Evaristo L.
Palabras clave : FSH
Gonadotropin
GnRH
LH
Reproductive dysfunction
Senegalese sole
Solea senegalensis
Spawning season
Fecha de publicación : 2-Mar-2009
Editor: Elsevier
Citación : Comparative Biochemistry and Physiology - A - Molecular and Integrative Physiology 153(3): 266-277 (2009)
Resumen: The Senegalese sole (Solea senegalensis) is a valuable flatfish for aquaculture, but it presents important reproductive problems in captivity. Spawning is achieved by wild-caught breeders but cultured broodstocks fail to spawn spontaneously and, when they do, eggs are unfertilized. To gain knowledge on the physiological basis underlying this reproductive dysfunction, this study aimed at analyzing comparative hormone levels between wild and cultured broodstocks at the spawning season. The Senegalese sole gonadotropin (GTH) subunits, FSHβ, LHβ and GPα, were cloned and qualitative (in situ hybridization) and quantitative (real-time PCR) assays developed to analyze pituitary GTH gene expression. In females, FSHβ and GPα mRNA levels were higher in wild than in cultured broodstocks, whereas in males all three subunits were highest in cultured. By ELISA, three GnRH forms were detected in the pituitary, displaying a relative abundance of GnRH2 > GnRH1 > GnRH3. All GnRHs were slightly more abundant in wild than cultured females, whereas no differences were observed in males. Plasma levels of vitellogenin and sex steroids were also analyzed. Results showed endocrine differences between wild and cultured broodstocks at the spawning period, which could be related to the endocrine failure of the reproductive axis in cultured breeders.
Descripción : 12 páginas, 8 figuras, 2 tablas.
Versión del editor: http://dx.doi.org/10.1016/j.cbpa.2009.02.032
URI : http://hdl.handle.net/10261/43064
ISSN: 1095-6433
DOI: 10.1016/j.cbpa.2009.02.032
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