Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/42638
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | A 48 SNP set for grapevine cultivar identification |
Autor: | Cabezas Martínez, José Antonio; Ibáñez Marcos, Javier CSIC ORCID ; Lijavetzky, Diego; Vélez, M. Dolores; Bravo, Gema; Rodríguez, Virginia; Carreño, Iván; Jermakow, Angelica M.; Carreño, Juan; Ruiz-García, Leonor; Thomas, Mark R.; Martínez-Zapater, José M. CSIC ORCID | Fecha de publicación: | 8-nov-2011 | Editor: | BioMed Central | Citación: | BMC Plant Biology 11(1): 153 (2011) | Resumen: | [Background]: Rapid and consistent genotyping is an important requirement for cultivar identification in many crop species. Among them grapevine cultivars have been the subject of multiple studies given the large number of synonyms and homonyms generated during many centuries of vegetative multiplication and exchange. Simple sequence repeat (SSR) markers have been preferred until now because of their high level of polymorphism, their codominant nature and their high profile repeatability. However, the rapid application of partial or complete genome sequencing approaches is identifying thousands of single nucleotide polymorphisms (SNP) that can be very useful for such purposes. Although SNP markers are bi-allelic, and therefore not as polymorphic as microsatellites, the high number of loci that can be multiplexed and the possibilities of automation as well as their highly repeatable results under any analytical procedure make them the future markers of choice for any type of genetic identification. [Results]: We analyzed over 300 SNP in the genome of grapevine using a re-sequencing strategy in a selection of 11 genotypes. Among the identified polymorphisms, we selected 48 SNP spread across all grapevine chromosomes with allele frequencies balanced enough as to provide sufficient information content for genetic identification in grapevine allowing for good genotyping success rate. Marker stability was tested in repeated analyses of a selected group of cultivars obtained worldwide to demonstrate their usefulness in genetic identification. [Conclusions]: We have selected a set of 48 stable SNP markers with a high discrimination power and a uniform genome distribution (2-3 markers/chromosome), which is proposed as a standard set for grapevine (Vitis vinifera L.) genotyping. Any previous problems derived from microsatellite allele confusion between labs or the need to run reference cultivars to identify allele sizes disappear using this type of marker. Furthermore, because SNP markers are bi-allelic, allele identification and genotype naming are extremely simple and genotypes obtained with different equipments and by different laboratories are always fully comparable. | Versión del editor: | http://dx.doi.org/10.1186/1471-2229-11-153 | URI: | http://hdl.handle.net/10261/42638 | DOI: | 10.1186/1471-2229-11-153 |
Aparece en las colecciones: | (CNB) Artículos (ICVV) Artículos (INIA) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
1471-2229-11-153.xml | 156,72 kB | XML | Visualizar/Abrir | |
1471-2229-11-153-S1.XLS | 212,5 kB | Microsoft Excel | Visualizar/Abrir | |
1471-2229-11-153.pdf | 473,29 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
40
checked on 02-abr-2024
SCOPUSTM
Citations
118
checked on 13-abr-2024
WEB OF SCIENCETM
Citations
108
checked on 26-feb-2024
Page view(s)
473
checked on 18-abr-2024
Download(s)
634
checked on 18-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
Artículos relacionados:
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.