Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/42602
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Distribution of Fitness Effects Caused by Single-Nucleotide Substitutions in Bacteriophage f1 |
Autor: | Peris, Joan B.; Davis, Paulina; Cuevas, José M. CSIC ORCID; Nebot Gómez, Miguel; Sanjuán, Rafael CSIC ORCID | Fecha de publicación: | jun-2010 | Editor: | Genetics Society of America | Citación: | Genetics 185 (2): 603-U308 (2010) | Resumen: | Empirical knowledge of the fitness effects of mutations is important for understanding many evolutionary processes, yet this knowledge is often hampered by several sources of measurement error and bias. Most of these problems can be solved using site-directed mutagenesis to engineer single mutations, an approach particularly suited for viruses due to their small genomes. Here, we used this technique to measure the fitness effect of 100 single-nucleotide substitutions in the bacteriophage f1, a filamentous single-strand DNA virus. We found that approximately one-fifth of all mutations are lethal. Viable ones reduced fitness by 11% on average and were accurately described by a log-normal distribution. More than 90% of synonymous substitutions were selectively neutral, while those affecting intergenic regions reduced fitness by 14% on average. Mutations leading to amino acid substitutions had an overall mean deleterious effect of 37%, which increased to 45% for those changing the amino acid polarity. Interestingly, mutations affecting early steps of the infection cycle tended to be more deleterious than those affecting late steps. Finally, we observed at least two beneficial mutations. Our results confirm that high mutational sensitivity is a general property of viruses with small genomes, including RNA and single-strand DNA viruses infecting animals, plants, and bacteria. | Versión del editor: | http://dx.doi.org/10.1534/genetics.110.115162 | URI: | http://hdl.handle.net/10261/42602 | DOI: | 10.1534/genetics.110.115162 | ISSN: | 0016-6731 |
Aparece en las colecciones: | (IFIC) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Embargo_Gral.pdf | 21,67 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
42
checked on 15-abr-2024
SCOPUSTM
Citations
51
checked on 23-abr-2024
WEB OF SCIENCETM
Citations
51
checked on 23-feb-2024
Page view(s)
355
checked on 22-abr-2024
Download(s)
174
checked on 22-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
Artículos relacionados:
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.