English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/42513
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:


The Stress-activated Protein Kinase Hog1 Mediates S Phase Delay in Response to Osmostress

AuthorsYaakov, Gilad; Duch, Alba; García-Rubio, María L. ; Clotet, Josep; Jiménez, Javier; Aguilera, Andrés ; Posas, Francesc
Cell cycle proteins
Osmotic pressure
Saccharomyces cerevisiae
DNA polymerase II
Cyclin B
Issue Date28-May-2009
PublisherAmerican Society for Cell Biology
CitationMolecular Biology of the Cell 20(15): 3572-3582 (2009)
AbstractControl of cell cycle progression by stress-activated protein kinases (SAPKs) is essential for cell adaptation to extracellular stimuli. Exposure of yeast to osmostress activates the Hog1 SAPK, which modulates cell cycle progression at G1 and G2 by the phosphorylation of elements of the cell cycle machinery, such as Sic1 and Hsl1, and by down-regulation of G1 and G2 cyclins. Here, we show that upon stress, Hog1 also modulates S phase progression. The control of S phase is independent of the S phase DNA damage checkpoint and of the previously characterized Hog1 cell cycle targets Sic1 and Hsl1. Hog1 uses at least two distinct mechanisms in its control over S phase progression. At early S phase, the SAPK prevents firing of replication origins by delaying the accumulation of the S phase cyclins Clb5 and Clb6. In addition, Hog1 prevents S phase progression when activated later in S phase or cells containing a genetic bypass for cyclin-dependent kinase activity. Hog1 interacts with components of the replication complex and delays phosphorylation of the Dpb2 subunit of the DNA polymerase. The two mechanisms of Hog1 action lead to delayed firing of origins and prolonged replication, respectively. The Hog1-dependent delay of replication could be important to allow Hog1 to induce gene expression before replication.
Description11 páginas, 8 figuras.
Publisher version (URL)http://dx.doi.org/10.1091/mbc.E09-02-0129
Appears in Collections:(CABIMER) Artículos
Files in This Item:
File Description SizeFormat 
ARTICULOS303405[1].pdf1,9 MBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.