Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/42470
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | R-loops do not accumulate in transcription-defective hpr1-101 mutants: implications for the functional role of THO/TREX |
Autor: | Gómez-González, Belén CSIC ORCID; Aguilera, Andrés CSIC ORCID | Palabras clave: | Alleles Cytidine deaminase Nuclear proteins Point mutation RNA-binding proteins Recombination Ribonuclease H Ribonucleaseproteins Saccharomyces cerevisiae proteins Transcription factors |
Fecha de publicación: | 18-may-2009 | Editor: | Oxford University Press | Citación: | Nucleic Acids Research 37(13): 4315-4321 (2009) | Resumen: | To get further insight into the effect that THO/TREX and R-loops have in transcription-associated recombination and transcription, we analyzed the ability to form R-loops of hpr1-101, a THO mutation that impairs transcription and mRNP biogenesis without triggering hyper-recombination. Human AID, a cytidine deaminase that acts on ssDNA displaced by RNA-DNA hybrids, strongly induced both hyper-recombination and hyper-mutation in hpr1-101, similar to hpr1Δ mutants. However, in contrast to hpr1Δ, AID-induced mutations in hpr1-101 occur at similar frequencies in both the transcribed and non-transcribed strands, implying that the enhanced AID action in these mutants is not caused by co-transcriptional R-loops. These results indicate for the first time that THO has a transcriptional function that is not mediated by R-loops, providing a new perspective for the understanding of the coupling of transcription with mRNP biogenesis and export. | Descripción: | 7 páginas, 6 figuras, 1 tabla. | Versión del editor: | http://dx.doi.org/10.1093/nar/gkp385 | URI: | http://hdl.handle.net/10261/42470 | DOI: | 10.1093/nar/gkp385 | ISSN: | 0305-1048 | E-ISSN: | 1362-4962 |
Aparece en las colecciones: | (CABIMER) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
ARTICULOS302979[1].pdf | 1,46 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
4
checked on 18-mar-2024
SCOPUSTM
Citations
9
checked on 15-mar-2024
WEB OF SCIENCETM
Citations
8
checked on 28-feb-2024
Page view(s)
403
checked on 17-mar-2024
Download(s)
81
checked on 17-mar-2024
Google ScholarTM
Check
Altmetric
Altmetric
Artículos relacionados:
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.