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Title

DNA-PK promotes the survival of young neurons in the embryonic mouse retina.

AuthorsBaleriola, Jimena ; Suárez, Teresa ; De la Rosa, Enrique J.
KeywordsDNA damage
DNA repair
Apoptosis
Programmed cell death
Neurogenesis
Neuronal differentiation
Double-strand break (DSB)
Retina
Nonhomologous end-joining (NHEJ)
Severe combined immunodeficiency (SCID)
Ataxia telangiectasia mutated (ATM)
Issue Date7-May-2010
PublisherNature Publishing Group
CitationCell Death and Differentiation 17(11):1697–1706(2010)
AbstractProgrammed cell death is a crucial process in neural development that affects mature neurons and glial cells, as well as proliferating precursors and recently born neurons at earlier stages. However, the regulation of the early phase of neural cell death and its function remain relatively poorly understood. In mouse models defective in homologous recombination or nonhomologous end-joining (NHEJ), which are both DNA double-strand break (DSB) repair pathways, there is massive cell death during neural development, even leading to embryonic lethality. These observations suggest that natural DSBs occur frequently in the developing nervous system. In this study, we have found that several components of DSB repair pathways are activated in the developing mouse retina at stages that coincide with the onset of neurogenesis. In short-term organotypic retinal cultures, we confirmed that the repair pathways can be modulated pharmacologically. Indeed, inhibiting the DNA-dependent protein kinase (DNA-PK) catalytic subunit, which is involved in NHEJ, with NU7026 increased caspase-dependent cell death and selectively reduced the neuron population. This observation concurs with an increase in the number of apoptotic neurons found after NU7026 treatment, as also observed in the embryonic scid mouse retina, a mutant that lacks DNA-PK catalytic subunit activity. Therefore, our results implicate the generation of DSB and DNA-PK-mediated repair in neurogenesis in the developing retina.
DescriptionSupplementary Information accompanies the paper on Cell Death and Differentiation website: http://www.nature.com/cdd/journal/v17/n11/suppinfo/cdd201046s1.html
Publisher version (URL)http://dx.doi.org/10.1038/cdd.2010.46
URIhttp://hdl.handle.net/10261/41986
DOI10.1038/cdd.2010.46
ISSN1350-9047
E-ISSN1476-5403
Appears in Collections:(CIB) Artículos
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