Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/41519
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Stabilization of Penicillin G Acylase from Escherichia coli: Site-Directed Mutagenesis of the Protein Surface To Increase Multipoint Covalent Attachment |
Autor: | Abian, Olga CSIC ORCID; Grazú, Valeria CSIC ORCID; Hermoso, Juan A. CSIC ORCID; González García, Ramón CSIC ORCID ; García, José Luis CSIC ORCID ; Fernández-Lafuente, Roberto CSIC ORCID; Guisán, José Manuel CSIC ORCID | Fecha de publicación: | feb-2004 | Editor: | American Society for Microbiology | Citación: | Applied and Environmental Microbiology 70(2):1249-1251 (2004) | Resumen: | Three mutations on the penicillin acylase surface (increasing the number of Lys in a defined area) were performed. They did not alter the enzyme's stability and kinetic properties; however, after immobilization on glyoxyl-agarose, the mutant enzyme showed improved stability under all tested conditions (e.g., pH 2.5 at 4°C, pH 5 at 60°C, pH 7 at 55°C, or 60% dimethylformamide), with stabilization factors ranging from 4 to 11 compared with the native enzyme immobilized on glyoxyl-agarose. | Versión del editor: | http://dx.doi.org/10.1128/AEM.70.2.1249-1251.2004 | URI: | http://hdl.handle.net/10261/41519 | DOI: | 10.1128/AEM.70.2.1249-1251.2004 | ISSN: | 0099-2240 |
Aparece en las colecciones: | (IFI) Artículos (IQF) Artículos (ICP) Artículos |
Mostrar el registro completo
CORE Recommender
PubMed Central
Citations
15
checked on 07-abr-2024
SCOPUSTM
Citations
105
checked on 23-abr-2024
WEB OF SCIENCETM
Citations
92
checked on 26-feb-2024
Page view(s)
373
checked on 21-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
Artículos relacionados:
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.