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Open Access item Hydroxylation of demethoxy-Q6 constitutes a control point in yeast coenzyme Q6 biosynthesis

Authors:Padilla, Sergio
Tran, U. C.
Jiménez-Hidalgo, María
López-Martín, José M.
Martín-Montalvo, Alejandro
Clarke, Catherine F.
Navas, Plácido
Santos-Ocaña, Carlos
Keywords:Ubiquinone, Coenzyme Q10, Mitochondria, Yeast regulation
Issue Date:11-Nov-2008
Publisher:Birkhäuser Verlag
Citation:Cellular and Molecular Life Sciences 66(1): 173-186 (2009)
Abstract:Coenzyme Q is a lipid molecule required for respiration and antioxidant protection. Q biosynthesis in Saccharomyces cerevisiae requires nine proteins (Coq1p–Coq9p). We demonstrate in this study that Q levels are modulated during growth by its conversion from demethoxy-Q (DMQ), a late intermediate. Similar conversion was produced when cells were subjected to oxidative stress conditions. Changes in Q6/DMQ6 ratio were accompanied by changes in COQ7 gene mRNA levels encoding the protein responsible for the DMQ hydroxylation, the penultimate step in Q biosynthesis pathway. Yeast coq null mutant failed to accumulate any Q late biosynthetic intermediate. However, in coq7 mutants the addition of exogenous Q produces the DMQ synthesis. Similar effect was produced by over-expressing ABC1/COQ8. These results support the existence of a biosynthetic complex that allows the DMQ6 accumulation and suggest that Coq7p is a control point for the Q biosynthesis regulation in yeast.
Description:23 páginas, 8 figuras, 2 tablas.
Publisher version (URL):http://dx.doi.org/10.1007/s00018-008-8547-7
E-ISSNmetadata.dc.identifier.doi = DOI:1420-9071
Appears in Collections:(CABD) Artículos

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