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Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/41492
Title: Hydroxylation of demethoxy-Q6 constitutes a control point in yeast coenzyme Q6 biosynthesis
Authors: Padilla, Sergio; Tran, U. C.; Jiménez-Hidalgo, María; López-Martín, José M.; Martín-Montalvo, Alejandro; Clarke, Catherine F.; Navas, Plácido; Santos-Ocaña, Carlos
Keywords: Ubiquinone
Coenzyme Q10
Yeast regulation
Issue Date: 11-Nov-2008
Publisher: Birkhäuser Verlag
Citation: Cellular and Molecular Life Sciences 66(1): 173-186 (2009)
Abstract: Coenzyme Q is a lipid molecule required for respiration and antioxidant protection. Q biosynthesis in Saccharomyces cerevisiae requires nine proteins (Coq1p–Coq9p). We demonstrate in this study that Q levels are modulated during growth by its conversion from demethoxy-Q (DMQ), a late intermediate. Similar conversion was produced when cells were subjected to oxidative stress conditions. Changes in Q6/DMQ6 ratio were accompanied by changes in COQ7 gene mRNA levels encoding the protein responsible for the DMQ hydroxylation, the penultimate step in Q biosynthesis pathway. Yeast coq null mutant failed to accumulate any Q late biosynthetic intermediate. However, in coq7 mutants the addition of exogenous Q produces the DMQ synthesis. Similar effect was produced by over-expressing ABC1/COQ8. These results support the existence of a biosynthetic complex that allows the DMQ6 accumulation and suggest that Coq7p is a control point for the Q biosynthesis regulation in yeast.
Description: 23 páginas, 8 figuras, 2 tablas.
Publisher version (URL): http://dx.doi.org/10.1007/s00018-008-8547-7
URI: http://hdl.handle.net/10261/41492
DOI: 10.1007/s00018-008-8547-7
ISSN: 1420-682X
E-ISSN: 1420-9071
References: PMC3070445
PMID: 19002377
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