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Título: | Morphological Changes in Autolytic Wine Yeast during Aging in Two Model Systems |
Autor: | Martínez-Rodríguez, Adolfo J. CSIC ORCID ; González García, Ramón CSIC ORCID ; Carrascosa, Alfonso V. CSIC ORCID | Palabras clave: | Autolysis Autophagy |
Fecha de publicación: | oct-2004 | Editor: | John Wiley & Sons | Citación: | Journal of Food Science 69(8): M233–M239 (2004) | Resumen: | Morphological changes produced in Saccharomyces cerevisiae IFI473 and 2 autolytic mutants derived from it (M1 and M2 mutants) were studied during yeast aging in 2 model systems (rich medium and model wine). Different conditions affecting autolysis, including temperature, culture media, nitrogen starvation, or phenyl-methylsulfonylfluoride addition, were analyzed. In rich medium, morphological changes mainly consisted in variation of cell size, presence of autophagic bodies inside the cytoplasm, detachment of the cytoplasm from the cell wall, spore formation, and loss of cytoplasmic material. Morphological changes were greater for mutant M2 than for the rest of the strains studied. In the wine medium, a decrease in cell size was the most relevant feature and the morphological changes observed were similar for all strains. Results obtained show morphological differences between autophagy and autolysis suggesting that yeast cells with accelerated autolysis could also present accelerated autophagy. | Versión del editor: | http://dx.doi.org/10.1111/j.1365-2621.2004.tb09893.x | URI: | http://hdl.handle.net/10261/41486 | DOI: | 10.1111/j.1365-2621.2004.tb09893.x | ISSN: | 0022-1147 | E-ISSN: | 1750-3841 |
Aparece en las colecciones: | (IFI) Artículos (ICVV) Artículos |
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