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dc.contributor.authorSilberstein, Lev-
dc.contributor.authorSánchez, María José-
dc.contributor.authorSocolovsky, Merav-
dc.contributor.authorLiu, Ying-
dc.contributor.authorHoffman, Gary-
dc.contributor.authorKinston, Sarah-
dc.contributor.authorPiltz, Sandie-
dc.contributor.authorBowen, Mark-
dc.contributor.authorGambardella, Laure-
dc.contributor.authorGreen, Anthony R.-
dc.contributor.authorGöttgens, Berthold-
dc.identifier.citationStem Cells 23(9): 1378-1388 (2005)es_ES
dc.description11 páginas, 5 figuras, 1 tabla.es_ES
dc.description.abstractAppropriate transcriptional regulation is critical for the biological functions of many key regulatory genes, including the stem cell leukemia (SCL) gene. As part of a systematic dissection of SCL transcriptional regulation, we have previously identified a 5,245-bp SCL +18/19 enhancer that targeted embryonic endothelium together with embryonic and adult hematopoietic progenitors and stem cells (HSCs). This enhancer is proving to be a powerful tool for manipulating hematopoietic progenitors and stem cells, but the design and interpretation of such transgenic studies require a detailed understanding of enhancer activity in vivo. In this study, we demonstrate that the +18/19 enhancer is active in mast cells, megakaryocytes, and adult endothelium. A 644-bp +19 core enhancer exhibited similar temporal and spatial activity to the 5,245-bp +18/19 fragment both during development and in adult mice. Unlike the +18/19 enhancer, the +19 core enhancer was only active in adult mice when linked to the eukaryotic reporter gene human placental alkaline phosphatase. Activity of a single core enhancer in HSCs, endothelium, mast cells, and megakaryocytes suggests possible overlaps in their respective transcriptional programs. Moreover, activity in a proportion of thymocytes and other SCL-negative cell types suggests the existence of a silencer elsewhere in the SCL locus.es_ES
dc.description.sponsorshipWork in the authors' laboratory is funded by the Leukaemia Research Fund, Wellcome Trust, BBSRC, and Cambridge MIT Institute. M.S. was supported by a National Cancer Institute Howard Temin (KO1) Award. M.J.S. was supported through an MRC career development award.es_ES
dc.publisherAlphaMed Presses_ES
dc.subjectHematopoietic stem celles_ES
dc.subjectTranscription factor SCL/tal-1es_ES
dc.subjectTranscriptional regulationes_ES
dc.subjectExperimental modelses_ES
dc.titleTransgenic analysis of the stem cell leukemia +19 stem cell enhancer in adult and embryonic hematopoietic and endothelial cellses_ES
dc.description.peerreviewedPeer reviewedes_ES
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