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Title: The Irx gene family in zebrafish: genomic structure, evolution and initial characterization of irx5b
Authors: Feijóo, Carmen Gloria; Manzanares, Miguel; Calle-Mustienes, Elisa de la; Gómez-Skarmeta, José Luis; Allende, Miguel L.
Keywords: Iroquois
Zebrafish
Development
Gene duplication
Teleost
Issue Date: 5-Apr-2004
Publisher: Springer
Citation: Development Genes and Evolution 214(6): 277-284 (2004)
Abstract: Genes of the iroquois (Iro/Irx) family are highly conserved from Drosophila to mammals and they have been implicated in a number of developmental processes. In flies, the Iro genes participate in patterning events in the early larva and in imaginal disk specification. In vertebrates, the Irx genes regulate developmental events during gastrulation, nervous system regionalization, activation of proneural genes and organ patterning. The Iro genes in Drosophila and the Irx genes of mammals show a clustered organization in the genome. Flies have a single cluster comprising three genes while mammals have two clusters also having three genes each. Moreover, experimental evidence in flies shows that transcriptional regulatory elements are shared among genes within the Iro cluster, suggesting that the same may be true in vertebrates. To date, the genomic organization of the Irx genes in non-mammalian species has not been studied. In this work, we have isolated the irx5b gene from zebrafish, Danio rerio, and have characterized its expression pattern. Furthermore, we have identified the complete set of Irx genes in two fish species, the zebrafish and pufferfish, Takifugu rubripes, and have determined the genomic organization of these genes. Our analysis indicates that early in fish evolutionary history, the Irx gene clusters have been duplicated and that subsequent events have maintained the clustered organization for some of the genes, while others have been lost. In total there are 11 existing Irx genes in zebrafish and 10 in pufferfish. We propose a new nomenclature for the zebrafish Irx genes based on the analysis of their sequences and their genomic relationships.
Description: 8 páginas, 4 figuras, 1 tabla. Electronic Supplementary Material Supplementary material is available in the online version of this article at http://dx.doi.org/10.1007/s00427-004-0401-9
Publisher version (URL): http://dx.doi.org/10.1007/s00427-004-0401-9
URI: http://hdl.handle.net/10261/41246
DOI: 10.1007/s00427-004-0401-9
ISSN: 0949-944X
E-ISSN: 1432-041X
References: PMID: 15064948
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