Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/41226
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Detection of Clostridium botulinum neurotoxin coding genes: analysis of PCR products by real time versus capillary gel electrophoresis methods |
Autor: | Sánchez-Hernández, Laura; Cifuentes, Alejandro CSIC ORCID ; Jiménez, Begoña CSIC ORCID ; Mateo, Rafael CSIC ORCID ; González García, Ramón CSIC ORCID | Palabras clave: | Capillary electrophoresis Clostridium botulinum |
Fecha de publicación: | 2008 | Editor: | Springer Nature | Citación: | European Food Research and Technology 227(2): 495-502 (2008) | Resumen: | In this work, two PCR-based methods have been developed for the detection of Clostridium botulinum strains carrying the gene coding for C. botulinum neurotoxin C (BoNTC) responsible for avian botulism. Both methods are based on the same amplification primers designed using multiple sequence alignments between toxin C coding sequences from DNA sequence databases. The first is a real-time PCR method, using a Taqman-MGB probe. The second uses conventional end-point PCR, followed by capillary gel electrophoresis with laser-induced fluorescence detection (CGE-LIF). A comparison between both methods has been established for the individual and simultaneous detection of toxin C (BONTC) or bacterial 16S (BACT) sequences from C. botulinum. The results indicate that, in general, the same sensitivity was achieved by using RT-PCR and PCR-CGE-LIF allowing the detection of both C. botulinum amplicons from concentrations as low as 7 × 10−5 μg/ml of total genomic DNA. Some other features from RT-PCR and CGE-LIF are also critically discussed in this work, including quantification capability, size determination, analysis speed and identification strategies, to provide enough information to adequately select the best analytical technique in each case. | Versión del editor: | http://dx.doi.org/10.1007/s00217-007-0746-1 | URI: | http://hdl.handle.net/10261/41226 | DOI: | 10.1007/s00217-007-0746-1 | ISSN: | 1438-2377 | E-ISSN: | 1438-2385 |
Aparece en las colecciones: | (IFI) Artículos (IREC) Artículos (IQOG) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 59,24 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
14
checked on 22-abr-2024
WEB OF SCIENCETM
Citations
14
checked on 29-feb-2024
Page view(s)
447
checked on 24-abr-2024
Download(s)
3
checked on 24-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.