English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/4098
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Characterization of two highly similar Rad51 homologs of Physcomitrella patens

AuthorsAyora, Silvia; Piruat, José I.; Luna, Rosa ; Reiss, Bernd; Russo, Vincenzo E. A.; Aguilera, Andrés ; Alonso, Juan Carlos
KeywordsDNA recombination
DNA repair
RecA
ATPases
Strand exchange
Issue Date8-Feb-2002
PublisherElsevier
CitationJ. Mol. Biol. 316, 35-49 (2002).
http://dx.doi.org/10.1006/jmbi.2001.5336
AbstractThe moss Physcomitrella patens, which is a land plant with efficient homologous recombination, encodes two Rad51 proteins (PpaRad51.1 and PpaRad51.2). The PpaRad51.1 and PpaRad51.2 proteins, which share 94 % identity between them, interact with themselves and with each other. Both proteins bind ssDNA and dsDNA in a Mg(2+) and pH-dependent manner, with a stoichiometry of one PpaRad51.1 monomer per 3(+/-1) nt or bp and one PpaRad51.2 monomer per 1(+/-0.5) nt or bp, respectively. At neutral pH, a 1.6-fold excess of both proteins is required for ssDNA and dsDNA binding. PpaRad51.1 and PpaRad51.2 show ssDNA-dependent ATPase activity and efficiently promote strand annealing in a nucleotide-independent but in a Mg(2+)-dependent manner. Both proteins promote joint-molecule formation, DNA strand invasion and are able to catalyse strand exchange in the presence of Mg(2+) and ATP. No further increase in the activities is observed when both proteins are present in the same reaction. None of the PpaRad51 gene products complement the DNA repair and recombination phenotype of Saccharomyces cerevisiae rad51delta mutants. However, PpaRad51.1 confers a dominant-negative DNA repair phenotype, and both PpaRad51 proteins reduce the levels of double-strand break-induced recombination when overexpressed in S. cerevisiae wt cells. These results suggest that both PpaRad51 proteins are bona fide Rad51 proteins that may contribute, in a different manner, to homologous recombination, and that they might replace ScRad51 in a hypothetical yeast protein complex inactivating different functions required for recombinational repair.
DescriptionPMID: 11829501.-- Final full-text version of the paper available at: http://www.sciencedirect.com/science/journal/00222836
URIhttp://hdl.handle.net/10261/4098
DOI10.1006/jmbi.2001.5336
ISSN0022-2836
Appears in Collections:(CNB) Artículos
(CABIMER) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.