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dc.contributor.authorTous, Cristina-
dc.contributor.authorAguilera, Andrés-
dc.date.accessioned2008-05-08T15:46:25Z-
dc.date.available2008-05-08T15:46:25Z-
dc.date.issued2007-08-24-
dc.identifier.citationBiochem Biophys Res Commun. 360(2): 428-432 (2007).en_US
dc.identifier.issn0006-291X-
dc.identifier.urihttp://hdl.handle.net/10261/4096-
dc.descriptionPMID: 17603014.-- Final version of the paper available at: http://www.sciencedirect.com/science/journal/0006291Xen_US
dc.description.abstractTranscription elongation causes a local change in DNA superhelicity. An excess of negative supercoiling may lead to opening of DNA strands that could allow formation of R-loops. In yeast, mutants of the THO complex are impaired in transcription elongation and this defect has been linked to co-transcriptional formation of R-loops, which could constitute roadblocks for RNA polymerases. In this study, we found that stably formed 300-nt long DNA–RNA hybrids in a negatively supercoiled transcription template reduced the efficiency of transcription elongation by half, providing a first experimental evidence that transcription elongation is impaired by R-loops in vitro.en_US
dc.description.sponsorshipThis work was supported by grants of the Spanish Ministry of Science and Education (GEN2001-4707-C08-02 and SAF2003-00204) and Junta de Andalucía (CVI102).en_US
dc.format.extent22195 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.rightsclosedAccessen_US
dc.subjectSaccharomyces cerevisiaeen_US
dc.subjectR-loopsen_US
dc.subjectTranscription elongationen_US
dc.subjectIn vitro transcriptionen_US
dc.subjectRNA polymerase IIen_US
dc.subjectDNA–RNA hybriden_US
dc.subjectRNAen_US
dc.titleImpairment of transcription elongation by R-loops in vitroen_US
dc.typeartículoen_US
dc.identifier.doi10.1016/j.bbrc.2007.06.098-
dc.description.peerreviewedPeer revieweden_US
dc.relation.publisherversionhttp://dx.doi.org/10.1016/j.bbrc.2007.06.098-
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