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Impairment of transcription elongation by R-loops in vitro

AuthorsTous, Cristina ; Aguilera, Andrés
KeywordsSaccharomyces cerevisiae
Transcription elongation
In vitro transcription
RNA polymerase II
DNA–RNA hybrid
Issue Date24-Aug-2007
CitationBiochem Biophys Res Commun. 360(2): 428-432 (2007).
AbstractTranscription elongation causes a local change in DNA superhelicity. An excess of negative supercoiling may lead to opening of DNA strands that could allow formation of R-loops. In yeast, mutants of the THO complex are impaired in transcription elongation and this defect has been linked to co-transcriptional formation of R-loops, which could constitute roadblocks for RNA polymerases. In this study, we found that stably formed 300-nt long DNA–RNA hybrids in a negatively supercoiled transcription template reduced the efficiency of transcription elongation by half, providing a first experimental evidence that transcription elongation is impaired by R-loops in vitro.
DescriptionPMID: 17603014.-- Final version of the paper available at: http://www.sciencedirect.com/science/journal/0006291X
Publisher version (URL)http://dx.doi.org/10.1016/j.bbrc.2007.06.098
Appears in Collections:(CABIMER) Artículos
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