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Closed Access item Functional Complementation in Yeast Allows Molecular Characterization of Missense Argininosuccinate Lyase Mutations

Authors:Trevisson, Eva
Burlina, Alberto
Doimo, Mara
Pertegato, Vanessa
Casarin, Alberto
Cesaro, Luca
Navas, Plácido
Basso, Giuseppe
Sartori, Geppo
Salviati, Leonardo
Keywords:Alleles, Argininosuccinate lyase, Genetic vectors, Genotype, Haploidy, Heterozygote, Phenotype, Saccharomyces cerevisiae, Urea
Issue Date:24-Aug-2009
Publisher:American Society for Biochemistry and Molecular Biology
Citation:The Journal of Biological Chemistry 284(): 28926-28934 (2009)
Abstract:Deficiency of argininosuccinate lyase (ASL) causes argininosuccinic aciduria, an urea cycle defect that may present with a severe neonatal onset form or with a late onset phenotype. To date phenotype-genotype correlations are still not clear because biochemical assays of ASL activity correlate poorly with clinical severity in patients. We employed a yeast-based functional complementation assay to assess the pathogenicity of 12 missense ASL mutations, to establish genotype-phenotype correlations, and to screen for intragenic complementation. Rather than determining ASL enzyme activity directly, we have measured the growth rate in arginine-free medium of a yeast ASL(null) strain transformed with individual mutant ASL alleles. Individual haploid strains were also mated to obtain diploid, "compound heterozygous" yeast. We show that the late onset phenotypes arise in patients because they harbor individual alleles retaining high residual enzymatic activity or because of intragenic complementation among different mutated alleles. In these cases complementation occurs because in the hybrid tetrameric enzyme at least one active site without mutations can be formed or because the differently mutated alleles can stabilize each other, resulting in partial recovery of enzymatic activity. Functional complementation in yeast is simple and reproducible and allows the analysis of large numbers of mutant alleles. Moreover, it can be easily adapted for the analysis of mutations in other genes involved in urea cycle disorders.
Description:9 páginas, 9 figuras, 2 tablas.
Publisher version (URL):http://dx.doi.org/10.1074/jbc.M109.050195
E-ISSNmetadata.dc.identifier.doi = DOI:1083-351X
Appears in Collections:(CABD) Artículos

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