English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/40645
Title: Genetic evidence for the requirement of the endocytic pathway in the uptake of coenzyme Q6 in Saccharomyces cerevisiae
Authors: Padilla, Sergio; Jiménez-Hidalgo, María; Martín-Montalvo, Alejandro; Clarke, Catherine F.; Navas, Plácido; Santos-Ocaña, Carlos
Keywords: Coenzyme Q
Q uptake
Mitochondrial disease
Issue Date: Jun-2009
Publisher: Elsevier
Citation: Biochimica et Biophysica Acta - Biomembranes 1788(6): 1238-1248 (2009)
Abstract: Coenzyme Q is an isoprenylated benzoquinone lipid that functions in respiratory electron transport and as a lipid antioxidant. Dietary supplementation with Q is increasingly used as a therapeutic for treatment of mitochondrial and neurodegenerative diseases, yet little is known regarding the mechanism of its uptake. As opposed to other yeast backgrounds, EG103 strains are unable to import exogenous Q(6) to the mitochondria. Furthermore, the distribution of exogenous Q(6) among endomembranes suggests an impairment of the membrane traffic at the level of the endocytic pathway. This fact was confirmed after the detection of defects in the incorporation of FM4-64 marker and CPY delivery to the vacuole. A similar effect was demonstrated in double mutant strains in Q(6) synthesis and several steps of endocytic process; those cells are unable to uptake exogenous Q(6) to the mitochondria and restore the growth on non-fermentable carbon sources. Additional data about the positive effect of peptone presence for exogenous Q(6) uptake support the hypothesis that Q(6) is transported to mitochondria through an endocytic-based system.
Description: 26 páginas, 7 figuras, 1 tabla.
Publisher version (URL): http://dx.doi.org/10.1016/j.bbamem.2009.03.018
URI: http://hdl.handle.net/10261/40645
DOI: 10.1016/j.bbamem.2009.03.018
ISSN: 0006-3002
References: PMC3070215
PMID: 19345667
Appears in Collections:(CABD) Artículos
Files in This Item:
File Description SizeFormat 
Genetic_2009.pdf1,99 MBAdobe PDFThumbnail
Show full item record

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.