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Closed Access item Transcription Activation or Repression by Phage Φ29 Protein p4 Depends on the Strength of the RNA Polymerase–Promoter Interactions

Authors:Monsalve, M.
Calles, Belén
Mencía, Mario
Salas, Margarita
Rojo, Fernando
Issue Date:Dec-1997
Publisher:Elsevier
Citation:Molecular Cell 1(1): 99-107 (1997)
Abstract:Phage Φ29 protein p4 activates the late A3 promoter and represses the early A2c promoter, in both cases by binding upstream from RNA polymerase (RNAP) and interacting with the C-terminal domain of the RNAP α subunit. To investigate how this interaction leads to activation at P A3 and to repression at P A2c, mutant promoters were constructed. We show that the position of protein p4 relative to that of RNAP, which is different at each promoter, does not dictate the outcome of the interaction. Rather, in the absence of a −35 consensus box for σA-RNAP activation was observed, while in its presence repression occurred. The results support the view that stabilization of RNAP at the promoter over a threshold level leads to repression.
Publisher version (URL):http://dx.doi.org/10.1016/S1097-2765(00)80011-8
URI:http://hdl.handle.net/10261/40590
ISSN:1097-2765
1097-4164
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Appears in Collections:(CBM) Artículos

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