Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/40549
Share/Impact:
Título : Characterization of the bacteriophage ø29-encoded protein p16.7: a membrane protein involved in phage DNA replication
Autor : Meijer, Wilhelmus J. J., Serna-Rico, A., Salas, Margarita
Fecha de publicación : 21-Dec-2001
Editor: John Wiley & Sons
Resumen: An early expressed operon, located at the right end of the linear bacteriophage φ29 genome, contains open reading frame (ORF)16.7, whose deduced protein sequence of 130 amino acids is conserved in φ29-related phages. Here, we show that this ORF actually encodes a protein, p16.7, which is abundantly and early expressed after infection. p16.7 is a membrane protein, and the N-terminally located transmembrane-spanning domain is required for its membrane localization. The variant p16.7A, in which the N-terminal membrane anchor was replaced by a histidine-tag, was purified and characterized. Purified p16.7A was shown to form dimers in solution. To study the in vivo role of p16.7, a φ29 mutant containing a suppressible mutation in gene 16.7 was constructed. In vivo phage DNA replication was affected in the absence of p16.7, especially at early infection times. Based on the results, the putative role of p16.7 in in vivoφ29 DNA replication is discussed.
Versión del editor: http://dx.doi.org/10.1046/j.1365-2958.2001.02260.x
URI : http://hdl.handle.net/10261/40549
ISSN: 0950-382X
DOI: 10.1046/j.1365-2958.2001.02260.x
Citación : Molecular Microbiology 39(3):731–746 (2001)
Appears in Collections:(CBM) Artículos

Files in This Item:
There are no files associated with this item.
Show full item record
 
CSIC SFX LinksSFX Query


Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.