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Título : A cloning strategy to obtain recombinant proteins with identical primary structure to the native forms
Autor : Santana, Margarida, Portillo Guisado, María del Carmen, González Grau, Juan Miguel
Palabras clave : Cloning strategy
Expression vectors
Native protein; Recombinant protein
Fusion tag
Biocatalysts
Fecha de publicación : Aug-2010
Editor: Elsevier
Resumen: Recombinant gene strategies using fusion tags for purification are essential procedures to obtain large protein quantities. However, most cloning systems result in recombinant proteins with added amino acids inexistent in their native forms which can lead to significant changes in protein properties. An original and simple cloning strategy is proposed to obtain proteins identical in amino acid sequence to the native proteins.
Descripción : 3 pages, 1 figure, 1 table, 4 references.
Versión del editor: http://dx.doi.org/doi:10.1016/j.jbiotec.2010.06.010
URI : http://hdl.handle.net/10261/40518
ISSN: 0168-1656
DOI: 10.1016/j.jbiotec.2010.06.010
Citación : Journal of Biotechnology 149 (1-2): 21-23 (2010)
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