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Open Access item A cloning strategy to obtain recombinant proteins with identical primary structure to the native forms

Authors:Santana, Margarida
Portillo Guisado, María del Carmen
González Grau, Juan Miguel
Keywords:Cloning strategy, Expression vectors, Native protein; Recombinant protein, Fusion tag, Biocatalysts
Issue Date:Aug-2010
Citation:Journal of Biotechnology 149 (1-2): 21-23 (2010)
Abstract:Recombinant gene strategies using fusion tags for purification are essential procedures to obtain large protein quantities. However, most cloning systems result in recombinant proteins with added amino acids inexistent in their native forms which can lead to significant changes in protein properties. An original and simple cloning strategy is proposed to obtain proteins identical in amino acid sequence to the native proteins.
Description:3 pages, 1 figure, 1 table, 4 references.
Publisher version (URL):http://dx.doi.org/doi:10.1016/j.jbiotec.2010.06.010
Appears in Collections:(IRNAS) Artículos

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