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Título: | Activation of Ras and Rho GTPases and MAP Kinases by G-Protein-Coupled Receptors |
Autor: | Chiariello, Mario; Vaqué, José P. CSIC ORCID; Crespo, Piero CSIC ORCID; Gutkind, J. Silvio | Palabras clave: | Kinase assays Western blot Phosphorylation Synthetic ligands RASSLs ERKs GTP Ras |
Fecha de publicación: | 2010 | Editor: | Springer Nature | Citación: | MAP Kinase Signaling Protocols: 137-150 (2010) | Serie: | Methods in Molecular Biology 661 |
Resumen: | A complex intracellular signaling network mediates the multiple biological activities of G-protein-coupled receptors (GPCRs). Among them, monomeric GTPases and a family of closely related proline-targeted serine–threonine kinases, collectively known as Mitogen-Activated Protein Kinases (MAPKs), appears to play central roles in orchestrating the proliferative responses to multiple mitogens that act on GPCRs. Upon GDP/GTP exchange, monomeric GTPases control the phosphorylation of conserved threonine and tyrosine residues in MAPKs by their immediate upstream kinases, increasing their enzymatic activity and inducing their translocation to the nucleus where they phosphorylate transcription factors, thereby regulating the expression of genes playing a key role in normal and aberrant cell growth. Recently, a number of GPCRs have been engineered to provide exclusive activation by synthetic drug-like compounds while becoming insensitive to endogenous ligands. These engineered receptors, named Receptors Activated Solely by Synthetic Ligands (RASSLs), promise better understanding of GPCRs signaling in vitro and in vivo, thus representing ideal tools to selectively modulate MAPK signaling routes controlling a wide range of biological functions, from proliferation to differentiation, migration, invasion, and cell survival or death by apoptosis. | Descripción: | 14 páginas, 2 figuras. | Versión del editor: | http://dx.doi.org/10.1007/978-1-60761-795-2_8 | URI: | http://hdl.handle.net/10261/40502 | DOI: | 10.1007/978-1-60761-795-2_8 | ISBN: | 978-1-60761-794-5 |
Aparece en las colecciones: | (IBBTEC) Libros y partes de libros |
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