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dc.contributor.authorNuez, Beatriz-
dc.contributor.authorRojo, Fernando-
dc.contributor.authorSalas, Margarita-
dc.date.accessioned2011-09-27T10:34:13Z-
dc.date.available2011-09-27T10:34:13Z-
dc.date.issued1992-12-01-
dc.identifier.citationProceedings of the National Academy of Sciences of the USA 89 (23) 11401-11405 (1992)es_ES
dc.identifier.issn1091-6490-
dc.identifier.urihttp://hdl.handle.net/10261/40096-
dc.description.abstractTranscription from the late promoter, PA3, of Bacillus subtilis phage phi 29 is activated by the viral regulatory protein p4. A kinetic analysis of the activation process has revealed that the role of protein p4 is to stabilize the binding of RNA polymerase to the promoter as a closed complex without significantly affecting further steps of the initiation process. Electrophoretic band-shift assays performed with a DNA fragment spanning only the protein p4 binding site showed that RNA polymerase could efficiently retard the complex formed by protein p4 bound to the DNA. Similarly, when a DNA fragment containing only the RNA polymerase-binding region of PA3 was used, p4 greatly stimulated the binding of RNA polymerase to the DNA. These results strongly suggest that p4 and RNA polymerase contact each other at the PA3 promoter. In the light of current knowledge of the p4 activation mechanism, we propose that direct contacts between the two proteins participate in the activation process.es_ES
dc.description.sponsorshipThis investigation has been aided by Research Grant SRO1 GM27242-13 from the National Institutes of Health, by Grant PB90-0091 from Direcci6n General de Investigaci6n Cientfifca y Tecnica, and by an Institutional Grant from Fundaci6n Ram6n Areces. B.N. and F.R. were holders of pre- and postdoctoral fellowships, respectively, from Ministerio de Educaci6n y Cienciaes_ES
dc.language.isoenges_ES
dc.publisherNational Academy of Sciences (U.S.)es_ES
dc.rightsopenAccesses_ES
dc.titlePhage phi 29 regulatory protein p4 stabilizes the binding of the RNA polymerase to the late promoter in a process involving direct protein-protein contactses_ES
dc.typeartículoes_ES
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://www.pnas.org/content/89/23/11401.shortes_ES
dc.contributor.funderNational Institutes of Health (US)-
dc.contributor.funderMinisterio de Economía y Competitividad (España)-
dc.contributor.funderFundación Ramón Areces-
dc.contributor.funderMinisterio de Educación y Ciencia (España)-
dc.identifier.funderhttp://dx.doi.org/10.13039/100000002es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/100008054es_ES
dc.type.coarhttp://purl.org/coar/resource_type/c_6501es_ES
item.openairetypeartículo-
item.cerifentitytypePublications-
item.fulltextWith Fulltext-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextopen-
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