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Closed Access item Transcription regulation in Bacillus subtilis phage Φ29
|Keywords:||Bacillus subtilis, DNA, Bacteriophage phi29, Transcription'|
|Citation:||Research in Microbiology 142(7-8):771-777(1991)|
|Abstract:||The molecular biology of Bacillus subtllis phage
e~29 has been extensively studied and now stands
among the best known phages infecting Grampositive
bacteria. As a resait, it has become a model
system for the study of proteln-primed DNA replication
(Salas, 1991)and phage particle morphogenesis
(Casjens and Hendrlx, 1988). The tight control
in the expression of the different genes involved in
these two processes also make it an interesting model
for the study of transcription regulation. At early
periods of infection, only the genes involved in DNA
replication and transcription regulation are expressed.
Genes coding for structural components of
the phage particle, or for proteins involved in morphogenesis
and cell lysis, are transcribed at later
stages of infection, when DNA replication is well advanced.
Early genes are all coded by the same DNA
strand and are located at both ends of the genome
(lneiarte el al,, 1976; Mellado et al., 1976; see fig. l).
Late genes are coded by the complementary strand
and are clustered in the central part of the genome,
a linear molecule 19285 bp long (Vl,~ek and Paces,
1986) witi~ the viral terminal protein p3 covalemly
linked at both 5' ends (Salas et at., 1978).|
|Publisher version (URL):||http://dx.doi.org/10.1016/0923-2508(91)90054-E|
|Appears in Collections:||(CBM) Artículos|
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