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Open Access item Towards industrially-feasible delignification and pitch removal by treating paper pulp with Myceliophthora thermophila laccase and a phenolic mediator

Authors:Babot, Esteban Daniel
Rico Campos, Alejandro
Rencoret, Jorge
Kalum, Lisbeth
Lund, Henrik
Romero Sánchez, Javier
Río Andrade, José Carlos del
Martínez, Ángel T.
Gutiérrez Suárez, Ana
Keywords:Laccase, Methyl syringate, Pitch deposits, Pulp bleaching, Syringaldehyde
Issue Date:2011
Publisher:Elsevier
Citation:Bioresource Technology 102: 6717-6722 (2011)
Abstract:The ability of two natural phenols to act as mediators of the recombinant Myceliophthora thermophila laccase (MtL) in eucalypt-pulp delignification was investigated. After alkaline peroxide extraction, the properties of the enzymatically-treated pulps improved with respect to the control. The pulp brightness increased (3.1 points) after the enzymatic treatment with MtL alone, but the highest improvements were obtained after the MtL treatment using syringaldehyde (4.7 points) and especially methyl syringate (8.3 points) as mediators. Likewise, a decrease in kappa number up to 2.7 points was obtained after the MtL–methyl syringate treatment, followed by decreases of 1.4 and 0.9 points after the treatments with MtL–syringaldehyde and MtL alone, respectively. On the other hand, removal of the main lipophilic extractives present in eucalypt pulp was observed after the above laccase–mediator treatments. Finally, the doses of both MtL and methyl syringate were reduced, and results compatible with industrial implementation were obtained.
URI:http://hdl.handle.net/10261/39997
???metadata.dc.identifier.doi???:http://dx.doi.org/10.1016/j.biortech.2011.03.100
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