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Open Access item Cloning and characterization of the gene encoding a blastieidin S acetyltnnfm from Streptoverticillum sp.
|Authors:||Pérez-González, José A.|
Esteban, J. A.
Jiménez Díaz, Antonio
|Keywords:||Recombinant DNA, Streptomyces, antibiotic resistance, plasmid vector, nucleotide sequence, promoter activity|
|Citation:||Gene 86 (1990) 129-134|
|Abstract:||A gene (b/s) encoding a blasticidin $-acetyltransferase from $~ptovenfcillum sp. JCM4673 was cloned in the Sweptomyces
plasmid plJ702 and expressed in 8. lividans 1326. Four ofthe recombinant plasmids contained a common 1129-bp Bcll-Psti
DNA fragment, which by subcloning experiments was shown to carry the b/.v gene. Sequencing of the 1129-bp Bcll.Pstl
fragment indicated that it contains two open reading frames of 408 and 366 nucleotides (nt), both starting with ATG and
terndnating at TGA triplets. Deletion analysis showed that the correct b/s sequence is that of 408 nt, whose deduced amino
acid sequence conforms to a polypeptide of Mr 15073. A 175-bp DNA fragment from the 5' region of the b/s gene had
promoter activity in the promoter-probe plasmid plJ486.|
|Publisher version (URL):||http://www.sciencedirect.com/science/article/pii/037811199090125B|
|Appears in Collections:||(CBM) Artículos|
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