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Título: | In vitro DNA synthesis reactions using phi29 DNA polymerase and a DNA fragment encoding said polymerase |
Autor: | Blanco, Luis CSIC ORCID ; Bernad, Antonio CSIC ORCID; Salas, Margarita CSIC ORCID | Fecha de publicación: | 24-feb-1993 | Citación: | Publicación nr.: EP0527728 | Resumen: | An improved method for determining the nucleotide base sequence of a DNA molecule. The method includes annealing the DNA molecule with a primer molecule able to hybridize to the DNA molecule; incubating the annealed mixture in a vessel containing four different deoxynucleoside triphosphates, a DNA polymerase, and one or more DNA synthesis terminating agents which terminate DNA synthesis at a specific nucleotide base, wherein each the agent terminates DNA synthesis at a different nucleotide base; and separating the DNA products of the incubating reaction according to size, whereby at least a part of the nucleotide base sequence of the DNA can be determined. The improvement is provision of a DNA-polymerase which is a phi 29-type DNA polymerase. | Descripción: | Titular: Consejo Superior de Investigaciones Científicas (CSIC) | URI: | http://hdl.handle.net/10261/39780 |
Aparece en las colecciones: | (CBM) Patentes |
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EP0527728A1.pdf | 19,54 kB | Adobe PDF | Visualizar/Abrir |
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