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Open Access item φ29 DNA polymerase

Authors:Blanco, Luis
Bernad, Antonio
Salas, Margarita
Issue Date:19-Nov-1996
Citation:Publicación nr.: US 5576204
Abstract:An improved method for determining the nucleotide base sequence of a DNA molecule employs a φ-29 type DNA polymerase modified to have reduced or no exonuclease activity. The method includes annealing the DNA molecule with a primer molecule able to hybridize to the DNA molecule; incubating the annealed mixture in a vessel containing four different deoxynucleoside triphosphates, a DNA polymerase, and one or more DNA synthesis terminating agents which terminate DNA synthesis at a specific nucleotide base, wherein each the agent terminates DNA synthesis at a different nucleotide base; and separating the DNA products of the incubating reaction according to size, whereby at least a part of the nucleotide base sequence of the DNA can be determined. The improvement is provision of a DNA polymerase which is a φ29-type DNA polymerase.
Description:Titular: Consejo Superior Investigaciones Cientificas (CSIC)
Publisher version (URL):http://www.patentlens.net/patentlens/patents.html?patnums=US_5576204_A#tab_1
Appears in Collections:(CBM) Patentes

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