English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/39772
Title: φ29 DNA polymerase
Authors: Blanco, Luis; Bernad, Antonio; Salas, Margarita
Issue Date: 19-Nov-1996
Citation: Publicación nr.: US 5576204
Abstract: An improved method for determining the nucleotide base sequence of a DNA molecule employs a φ-29 type DNA polymerase modified to have reduced or no exonuclease activity. The method includes annealing the DNA molecule with a primer molecule able to hybridize to the DNA molecule; incubating the annealed mixture in a vessel containing four different deoxynucleoside triphosphates, a DNA polymerase, and one or more DNA synthesis terminating agents which terminate DNA synthesis at a specific nucleotide base, wherein each the agent terminates DNA synthesis at a different nucleotide base; and separating the DNA products of the incubating reaction according to size, whereby at least a part of the nucleotide base sequence of the DNA can be determined. The improvement is provision of a DNA polymerase which is a φ29-type DNA polymerase.
Description: Titular: Consejo Superior Investigaciones Cientificas (CSIC)
Publisher version (URL): http://www.patentlens.net/patentlens/patents.html?patnums=US_5576204_A#tab_1
URI: http://hdl.handle.net/10261/39772
Appears in Collections:(CBM) Patentes
Files in This Item:
File Description SizeFormat 
5576204_phi_29_DNA_polymerase (1).pdf237,72 kBAdobe PDFThumbnail
Show full item record

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.