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Open Access item In vitro evolution of terminal protein-containing genomes

Authors:Esteban, José A.
Blanco, Luis
Villar, Laurentino
Salas, Margarita
Keywords:φ29 DNA replication, DNA amplification
Issue Date:1-Apr-1997
Publisher:National Academy of Sciences (U.S.)
Citation:Proceedings of the National Academy of Sciences of the USA 94(7): 2921-2926 (1997)
Abstract:A new self-sustained terminal protein-primed DNA amplification system has been used to describe in vitro evolutionary changes affecting maintenance of the genome size of bacteriophage φ29. These changes involve generation and efficient amplification of short palindromic molecules containing an inverted duplication of one of the original DNA ends. A template-switching mechanism is proposed to account for the appearance of these molecules. After their formation, they would replicate by means of hairpin intermediates. Relevant kinetic information about this DNA replication system has been obtained from the competition between the input full-length φ29 DNA and its derived truncated versions. The physiological relevance of these molecules and the mechanisms to control their formation are discussed.
Publisher version (URL):http://www.pnas.org/content/94/7/2921.full.pdf
E-ISSNmetadata.dc.identifier.doi = DOI:1091-6490
Appears in Collections:(CBM) Artículos

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