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Open Access item The main early and late promoters of Bacillus subtilis phage ø29 form unstable open complexes with sA-RNA polymerase that are stabilized by DNA supercoiling

Authors:Rojo, Fernando
Nuez, Beatriz
Mencía, Mario
Salas, Margarita
Issue Date:1993
Publisher:Oxford University Press
Citation:Nucleic Acids Research 21(4): 935-940 (1993)
Abstract:Most Escherichia coli promoters studied so far form stable open complexes with σ70-RNA polymerase which have relatively long half-lives and, therefore, are resistant to a competitor challenge. A few exceptions are nevertheless known. The analysis of a number of promoters in Bacillus subtilis has suggested that the instability of open complexes formed by the vegetative σA polymerase may be a more general phenomenon than in Escherichia coli. We show that the main early and late promoters from the Bacillus subtilis phage ø29 form unstable open complexes that are stabilized either by the formation of the first phosphodlester bond between the initiating nucleoside triphosphates or by DNA supercoiling. The functional characteristics of these two strong promoters suggest that they are not optimized for a tight and stable RNA polymerase binding. Their high activity is probably the consequence of the efficiency of further steps leading to the formation of an elongation complex.
Publisher version (URL):http://dx.doi.org/10.1093/nar/21.4.935
URI:http://hdl.handle.net/10261/39587
ISSN:0305-1048
1362-4962
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