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Open Access item Activation of S1 nuclease at neutral pH

Authors:Esteban, José A.
Salas, Margarita
Blanco, Luis
Keywords:S1 nuclease
Issue Date:17-Jul-1992
Publisher:Oxford University Press
Citation:Nucleic Acids Research 20(18): 3409–341(2008)
Abstract:SI nuclease is a single-strand-specific endonuclease that degrades DNA and RNA to nucleoside 5'-monophosphates. It has an acid pH optimum (4.0-4.5) and requires Zn2+ or Co2+ for maximal activity (1). This enzyme is widely used in DNA manipulation, mainly for the characterization of mRNAs (SI-mapping) (2) and the study of the tertiary structure of DNA (3, 4). A distinct disadvantage of the SI nuclease is that it is active only at low pH.
Publisher version (URL):http://www.ncbi.nlm.nih.gov/pmc/articles/PMC334256/
E-ISSNmetadata.dc.identifier.doi = DOI:1362-4962
Appears in Collections:(CBM) Artículos

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