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Título : Activation of S1 nuclease at neutral pH
Autor : Esteban, José A., Salas, Margarita, Blanco, Luis
Palabras clave : S1 nuclease
Fecha de publicación : 17-Jul-1992
Editor: Oxford University Press
Resumen: SI nuclease is a single-strand-specific endonuclease that degrades DNA and RNA to nucleoside 5'-monophosphates. It has an acid pH optimum (4.0-4.5) and requires Zn2+ or Co2+ for maximal activity (1). This enzyme is widely used in DNA manipulation, mainly for the characterization of mRNAs (SI-mapping) (2) and the study of the tertiary structure of DNA (3, 4). A distinct disadvantage of the SI nuclease is that it is active only at low pH.
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ISSN: 0305-1048
Citación : Nucleic Acids Research 20(18): 3409–341(2008)
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