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Closed Access item A DNA curvature can substitute phage ø29 regulatory protein p4 when acting as a transcriptional repressor

Authors:Rojo, Fernando
Salas, Margarita
Keywords:Bacillus subtilis, Divergent promoter
Issue Date:1991
Publisher:Nature Publishing Group
Citation:Embo Journal 10(11): 3429-3438 (1991)
Abstract:Binding of phage phi 29 regulatory protein p4 to its target sequences produces a strong bend in the DNA that is important for activation of the late A3 promoter (PA3). Protein p4 binding site in PA3 overlaps with the divergently transcribed main early promoter. PA2b, which suggested that p4 could also act as a repressor. We show that protein p4 both excludes Bacillus subtilis sigma A-RNA polymerase from PA2b and directs it to the divergently transcribed A3 promoter. Although steric hindrance is likely to be involved in the repression process, we have also analysed the effect on PA2b activity of a sequence-dependent curvature that simulates that induced by protein p4. A progressive increase in the DNA curvature of protein p4 binding region, performed by site-directed mutagenesis, has indicated that a static DNA curvature by itself can inhibit transcription from PA2b, both by impairing the binding of sigma A-RNA polymerase to the promoter and by reducing its ability to form transcriptionally active open complexes. These results indicate that bending promoter sequences in a direction unfavourable for RNA polymerase binding can repress transcription. Protein p4-induced DNA bending could therefore participate in PA2b repression by producing a DNA structure not recognized as a promoter by sigma A-RNA polymerase.
Description:PMCID: PMC453071
Publisher version (URL):http://www.ncbi.nlm.nih.gov/pmc/articles/PMC453071/
URI:http://hdl.handle.net/10261/39577
ISSN:0261-4189
E-ISSNmetadata.dc.identifier.doi = DOI:1460-2075
Appears in Collections:(CBM) Artículos

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