Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/39577
Título : A DNA curvature can substitute phage ø29 regulatory protein p4 when acting as a transcriptional repressor
Autor : Rojo, Fernando, Salas, Margarita
Palabras clave : Bacillus subtilis
Divergent promoter
Fecha de publicación : 1991
Editor: Nature Publishing Group
Citación : Embo Journal 10(11): 3429-3438 (1991)
Resumen: Binding of phage phi 29 regulatory protein p4 to its target sequences produces a strong bend in the DNA that is important for activation of the late A3 promoter (PA3). Protein p4 binding site in PA3 overlaps with the divergently transcribed main early promoter. PA2b, which suggested that p4 could also act as a repressor. We show that protein p4 both excludes Bacillus subtilis sigma A-RNA polymerase from PA2b and directs it to the divergently transcribed A3 promoter. Although steric hindrance is likely to be involved in the repression process, we have also analysed the effect on PA2b activity of a sequence-dependent curvature that simulates that induced by protein p4. A progressive increase in the DNA curvature of protein p4 binding region, performed by site-directed mutagenesis, has indicated that a static DNA curvature by itself can inhibit transcription from PA2b, both by impairing the binding of sigma A-RNA polymerase to the promoter and by reducing its ability to form transcriptionally active open complexes. These results indicate that bending promoter sequences in a direction unfavourable for RNA polymerase binding can repress transcription. Protein p4-induced DNA bending could therefore participate in PA2b repression by producing a DNA structure not recognized as a promoter by sigma A-RNA polymerase.
Descripción : PMCID: PMC453071
Versión del editor: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC453071/
URI : http://hdl.handle.net/10261/39577
ISSN: 0261-4189
Appears in Collections:(CBM) Artículos

Files in This Item:
There are no files associated with this item.
Show full item record
 
CSIC SFX LinksSFX Query

Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.