Cellular Approach to the Study of Androgenesis in Maize Anthers: lmrnunocytochemical Evidence of the lnvolvernent of the Ubiquitin Degradative Pathway in Androgenesis lnduction

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Título:	Cellular Approach to the Study of Androgenesis in Maize Anthers: lmrnunocytochemical Evidence of the lnvolvernent of the Ubiquitin Degradative Pathway in Androgenesis lnduction
Autor:	Alché Ramírez, Juan de Dios CSIC ORCID; Castro López, Antonio Jesús CSIC ORCID; Rodríguez García, María I. CSIC ORCID
Palabras clave:	Androgenesis Immunocytochemistry ubiquitin Ultrastructure Zea mays L.
Fecha de publicación:	2000
Editor:	Urban and Fischer
Citación:	Journal of plant physiology 156: 146-155 (2000)
Resumen:	Maize anthers in the late-uninucleare stage, corresponding to a DH 109 x ZK 191/a F1 hybrid line, were induced to produce embryoids by cold pre-treatment and incubation onto a modified YP medium. LM observation of sections of the anthers showed the existente of bicellular microspores two days after starting the culture. Several types of multicellular microspores (MCM) and multinucleate microspores (MNM) appeared from the fifth day after culture onwards, together with many degenerated, non-induced microspores. This abundante of forms reveals the presence of different patterns of division and development. Ultrastructural observation of these cellular forms showed particular features, such as the presence of numerous nuclear pores in early stages of induction and the formation of a thick independent cell wall underneath the microspore intine. A certain degree of polarity in these structures was also frequently observed. Proembryoids appeared after 10-13 days of culture, whereas embryo-like structures were observed several days later (16 days of culture).
Descripción:	11 páginas, 5 figuras.-- et al.
URI:	http://hdl.handle.net/10261/39511
Aparece en las colecciones:	(EEZ) Artículos