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Título : Dynamic relocalization of phage ø29 DNA during replication and the role of the viral protein
Autor : Meijer, Wilhelmus J. J., Lewis, Peter J., Errington, Jeff, Salas, Margarita
Palabras clave : Bacillus subtilis
Bacteriophage pi29
In vivo DNA replication
Fecha de publicación : 1-Aug-2000
Editor: Nature Publishing Group
Resumen: We have examined the localization of DNA replication of the Bacillus subtilis phage 29 by immunofluorescence. To determine where phage replication was localized within infected cells, we examined the distribution of phage replication proteins and the sites of incorporation of nucleotide analogues into phage DNA. On initiation of replication, the phage DNA localized to a single focus within the cell, nearly always towards one end of the host cell nucleoid. At later stages of the infection cycle, phage replication was found to have redistributed to multiple sites around the periphery of the nucleoid, just under the cell membrane. Towards the end of the cycle, phage DNA was once again redistributed to become located within the bulk of the nucleoid. Efficient redistribution of replicating phage DNA from the initial replication site to various sites surrounding the nucleoid was found to be dependent on the phage protein p16.7.
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ISSN: 0261-4189
DOI: 10.1093/emboj/19.15.4182
Citación : Embo Journal 19(15): 4182–4190 (2000)
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