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Open Access item Identification of the sequences recognized by phage ϕ29 transcriptional activator: possible interaction between the activator and the RNA polymerase

Authors:Nuez, Beatriz
Rojo, Fernando
Barthelemy, Isabel
Salas, Margarita
Issue Date:1991
Publisher:Oxford University Press
Citation:Nucleic Acids Research 19(9): 2337-2342 (1991)
Abstract:requires the transcriptional activator protein p4. This activator binds to a region of the late A3 promoter spanning nucleotides -56 to -102 relative to the transcription start site, generating a strong bendlngTIn the DNA. In this work the target sequences recognized by protein p4 In the phage φ29 late A3 promoter have been characterized. The binding of protein p4 to derivatives of the late A3 promoter harbouring deletions in the protein p4 binding site has been studied. When protein p4 recognition sequences were altered, the activator could only bind to the promoter in the presence of RNA polymerase. This strong cooperatlvtty in the binding of protein p4 and RNA polymerase to the promoter suggests the presence of direct protein-protein contacts between them.
Publisher version (URL):http://dx.doi.org/10.1093/nar/19.9.2337
E-ISSNmetadata.dc.identifier.doi = DOI:1362-4962
Appears in Collections:(CBM) Artículos

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