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Closed Access item Short N-terminal deletions in the phage ø29 transcriptional activator protein impair its DNA-binding ability

Authors:Rojo, Fernando
Salas, Margarita
Keywords:Gene regulation, Promoter recognition
Issue Date:1990
Citation:Gene 96(1): 75-81 (1990)
Abstract:The expression of Bacillus subtilis phage φ29 late genes from the A3 promoter requires the viral protein p4. This protein is a transcriptional activator which binds to a region of the A3 promoter located between nucleotides −56 to −102, relative to the transcription start point. Mutants at the N terminus of protein p4 have been constructed and their function investigated. The binding of these deletion mutants to the late A3 promoter has been analyzed by gel retardation and DNase I footprinting assays. The results indicate that the N terminus of protein p4 could be involved in its binding to the A3 promoter, suggesting that it may not be a typical Cro-like helix-turn-helix DNA-binding protein.
Publisher version (URL):http://dx.doi.org/10.1016/0378-1119(90)90343-P
Appears in Collections:(CBM) Artículos

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