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Título: | A set of expression plasmids for the synthesis of fused and unfused polypeptides in Escherichia coli |
Autor: | Zaballos, Ángel; Salas, Margarita CSIC ORCID ; Mellado, Rafael P. | Palabras clave: | ORF vectors Recombinant DNA Epitope mapping Influenza A virus |
Fecha de publicación: | 1987 | Editor: | Elsevier | Citación: | Gene 58(1): 67-76 (1987) | Resumen: | A set of plasmid expression vectors for cloning of DNA fragments containing open reading frames has been obtained. The plasmids carry the strong leftward promoter of bacteriophage λ and the translation initiation signals from either the gene ner of bacteriophage Mu or the gene 4 of bacteriophage 029. The vectors could overexpress the cloned sequences as fusion peptides at the N terminus with the N-terminal segment of the 029 protein p4 or at the C terminus with the Escherichia coli β-galactosidase from its 8th residue, or both. Alternatively, the cloned sequences could be directed to overproduce proteins in an unfused form. DNA fragments of the hemagglutinin gene from human influenza A virus, have been cloned in one of the plasmid vectors and some potential antigenic determinants have been characterized using monoclonal antibodies. | Versión del editor: | http://dx.doi.org/10.1016/0378-1119(87)90030-8 | URI: | http://hdl.handle.net/10261/39246 | DOI: | 10.1016/0378-1119(87)90030-8 | ISSN: | 0378-1119 |
Aparece en las colecciones: | (CBM) Artículos |
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