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Closed Access item A set of expression plasmids for the synthesis of fused and unfused polypeptides in Escherichia coli
Mellado, Rafael P.
|Keywords:||ORF vectors, Recombinant DNA, Epitope mapping, Influenza A virus|
|Citation:||Gene 58(1): 67-76 (1987)|
|Abstract:||A set of plasmid expression vectors for cloning of DNA fragments containing open reading frames has been obtained. The plasmids carry the strong leftward promoter of bacteriophage λ and the translation initiation signals from either the gene ner of bacteriophage Mu or the gene 4 of bacteriophage 029. The vectors could overexpress the cloned sequences as fusion peptides at the N terminus with the N-terminal segment of the 029 protein p4 or at the C terminus with the Escherichia coli β-galactosidase from its 8th residue, or both. Alternatively, the cloned sequences could be directed to overproduce proteins in an unfused form. DNA fragments of the hemagglutinin gene from human influenza A virus, have been cloned in one of the plasmid vectors and some potential antigenic determinants have been characterized using monoclonal antibodies.|
|Publisher version (URL):||http://dx.doi.org/10.1016/0378-1119(87)90030-8|
|Appears in Collections:||(CBM) Artículos|
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