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Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/39244
Title: A Bacillus subtilis phage φ29 transcription terminator is efficiently recognized in Streptomyces lvidans
Authors: Pulido, Diego; Jiménez Díaz, Antonio; Salas, Margarita; Mellado, Rafael P.
Keywords: Recombinant DNA
S1 mapping
RNA polymerase
Inverted repeat
Kanamycin resistance
Heterologous expression
Issue Date: 1987
Publisher: Elsevier
Citation: Gene 56(2-3): 277-282 (1987)
Abstract: A DNA fragment from the Bacillus subtilis phage φ29, containing the bidirectional transcription terminator TD1, where the right early transcription and late viral transcription terminate, has been inserted in one orientation between the aminoglycoside phosphotransferase (APH) gene (neo) and the φ29 main early and late promoters present in derivative constructs of the Streptomyces promoter-probe plasmid pIJ486. The TD1 terminator is efficiently recognized in S. lividans and ends the transcription, started in vivo at the φ29 promoters, at the same point as the B. subtilis RNA polymerase, resulting in a considerable reduction of the level of the APHII enzyme synthesized under the control of the phage promoters.
Description: Short communication
Publisher version (URL): http://dx.doi.org/10.1016/0378-1119(87)90144-2
URI: http://hdl.handle.net/10261/39244
DOI: 10.1016/0378-1119(87)90144-2
ISSN: 0378-1119
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