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Closed Access item A Bacillus subtilis phage φ29 transcription terminator is efficiently recognized in Streptomyces lvidans

Authors:Pulido, Diego
Jiménez Díaz, Antonio
Salas, Margarita
Mellado, Rafael P.
Keywords:Recombinant DNA, S1 mapping, RNA polymerase, Inverted repeat, Kanamycin resistance, Heterologous expression, Plasmids
Issue Date:1987
Citation:Gene 56(2-3): 277-282 (1987)
Abstract:A DNA fragment from the Bacillus subtilis phage φ29, containing the bidirectional transcription terminator TD1, where the right early transcription and late viral transcription terminate, has been inserted in one orientation between the aminoglycoside phosphotransferase (APH) gene (neo) and the φ29 main early and late promoters present in derivative constructs of the Streptomyces promoter-probe plasmid pIJ486. The TD1 terminator is efficiently recognized in S. lividans and ends the transcription, started in vivo at the φ29 promoters, at the same point as the B. subtilis RNA polymerase, resulting in a considerable reduction of the level of the APHII enzyme synthesized under the control of the phage promoters.
Description:Short communication
Publisher version (URL):http://dx.doi.org/10.1016/0378-1119(87)90144-2
Appears in Collections:(CBM) Artículos

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